Tangential flow filtration of wine.

Comparison of Diafiltration and Tangential Flow Filtration for Purification

Purpose. The study reports evaluation of different purification processes for removing surplus surfactant and formulating stable nanoparticle dispersions. Methods. Nanoparticle formulations prepared from poly(D,L-lactide-co-glycolide) and polyvinyl alcohol (PVA) were purified by a diafiltration centrifugal device (DCD), using 300K and 100K molecular weight cut-off (MWCO) membranes and a tangent...

Protein Concentration and Diafiltration by Tangential Flow Filtration

PURPOSE Membrane-based Tangential Flow Filtration (TFF) unit operations are used for clarifying, concentrating, and purifying proteins. This technical brief is a practical introduction to protein processing using tangential flow filtration. It is intended to help scientists and engineers achieve their protein processing objectives by discussing how the choice of key components and operating par...

Automation of Tangential Flow Filtration for Purification of Biosynthetic Spider Silk

Separation of Spermatozoa with a Combination of Pinched Flow Fractionation and Tangential Filtration

We demonstrate a pinched flow tangential filtration method to sort spermatozoa from larger particles with a spermatozoa collection efficiency of 94±2% and a separation efficiency of 100%. In conventional pinched flow fractionation (PFF), an observed tumbling-like rotation of spermatozoa complicates their separation from other particles. The new design circumvents this effect.

Enumeration and isolation of viral particles from oligotrophic marine environments by tangential flow filtration.

A method for concentrating, enumerating and isolating viral particles from marine water samples was developed and evaluated. The method consists of a concentration step by a tangential flow filtration (TFF) system, ultrafiltration by centrifugal concentrator, and visualization by transmission electron microscopy (TEM). This procedure allows to reduce volumes of ca. 21 of seawater to 10-20 micro...